The study was designed to investigate the cardioprotective and antioxidant effects of Tinospora cordifolia against isoproterenol induced myocardial damage in rats. Sixty, male wistar rats were randomly divided into five groups. Group 1 served as control and groups 2, 3, 4 and 5 were given isoproterenol @ 85 mg/kg b.wt. subcutaneously for four consecutive days. In addition, groups 3, 4 and 5 received vitamin E (60 mg/kg b.wt), T. cordifolia dose I (100 mg / kg b.wt) and dose II (200 mg / kg b.wt), respectively orally from day 1 till the end of experimental period. Whole blood and serum were collected on 5th, 8th and 12th days to estimate CPK, LDH, AST, ALT, serum troponins, whole blood GSH and erythrocyte antioxidant enzymes (GPx, GSH-R, and CAT). At the end of the experimental period, lipid peroxidation, gross and histopathology of heart was studied. Isoproterenol significantly (P < 0.05) elevated CPK, LDH, troponins and lipid peroxidation products of heart and reduced GSH, GPx, GSH-R and CAT activities, which were restored, to normal following treatment with Vitamin E and Tinospora cordifolia. Gross findings showed enlargement, pallor and ischemic changes of heart and histopathology revealed sarcolysis, vacuolation, hemorrhage, edema and infiltration of inflammatory cells in isoproterenol control, while mild lesions were noticed in treated groups. From the present study it is evident that T. cordifolia and Vitamin E could offer significant cardioprotective and antioxidant actions in isoproterenol induced cardiac damage. However, Vitamin E accomplished better action compared to T. cordifolia.
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